A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs
Braz. j. microbiol
;
40(4): 988-993, Oct.-Dec. 2009. ilus
Artículo
en Inglés
| LILACS
| ID: lil-528184
ABSTRACT
Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 mL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Virus ARN
/
Porcinos
/
Técnicas In Vitro
/
Genoma
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Virus de la Encefalomiocarditis
/
Nucleótidos
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Cuba
Institución/País de afiliación:
Grupo de Virología/CU
Similares
MEDLINE
...
LILACS
LIS