Serological diagnosis of Chagas disease: evaluation and characterisation of a low cost antigen with high sensitivity and specificity
Mem. Inst. Oswaldo Cruz
;
104(6): 914-917, Sept. 2009. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-529563
ABSTRACT
In spite of evident progress in the serology of Chagas disease, the requirement for new diagnostic antigens persists. We have evaluated different antigens obtained from Trypanosoma cruzi grown in medium rich in nutrients or under nutrient stress, autoclaved or sonicated and fractionated by differential centrifugation. The resulting antigens were evaluated for diagnosis of Chagas disease using ELISA. Immunofluorescence of the parasites demonstrated that nutrient stress induced changes in the distribution and density of antigens recognised by a pool of sera from experimentally infected mice. When evaluated using ELISA, it was evident that most fractions had good sensitivity but poor specificity. Surprisingly, the best specificity and sensitivity was observed with parasites cultured under nutrient stress and autoclaved. Furthermore this antigen had low cross reactivity with sera from other parasitic diseases, Leishmaniasis in particular. Western blot analysis demonstrated that autoclaving seems to non-specifically eliminate cross-reactive antigens. In conclusion, autoclaving epimastigotes of T. cruzi, after nutrient stress, allowed us to obtain an antigen that could be used in the serological diagnosis of Chagas disease.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma cruzi
/
Anticuerpos Antiprotozoarios
/
Enfermedad de Chagas
/
Antígenos de Protozoos
Tipo de estudio:
Estudio diagnóstico
/
Evaluación Económica en Salud
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2009
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Venezuela
Institución/País de afiliación:
Centro de Biotecnología/VE
/
Universidad Central de Venezuela/VE
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