Characterization of indigenous rhizobia from Caatinga
Braz. j. microbiol
;
41(1): 201-208, Jan.-Mar. 2010. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-531753
ABSTRACT
The aim of this study was to characterize rhizobial isolates from Cratylia mollis Mart. ex Benth, Calliandra depauperata Benth. and Mimosa tenuiflora (Willd.) Poir. by means of rhizobial colonies morphology and restriction analysis of the 16S ribosomal gene (16S rDNA-ARDRA). Nodules were collected in the field and from plants cultivated in a greenhouse experiment using Caatinga soil samples. Sixty seven isolates were described by morphological analysis. Forty seven representative isolates were used for ARDRA analysis using seven restriction enzymes. We observed high diversity of both slow and fast-growing rhizobia that formed three morpho-physiological clusters. A few fast-growing isolates formed a group of strains of the Bradyrhizobium type; however, most of them diverged from the B. japonicum and B. elkanii species. Cratylia mollis nodule isolates were the most diverse, while all Mimosa tenuiflora isolates displayed fast growth with no pH change and were clustered into groups bearing 100 percent similarity, according to ARDRA results.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Rhizobiaceae
/
Técnicas In Vitro
/
Activación Enzimática
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Centro Nacional de Pesquisa de Agrobiologia/BR
/
Fundação Instituto Brasileiro de Geografia e Estatística/BR
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