Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc. )
Braz. j. microbiol
;
41(1): 253-263, Jan.-Mar. 2010. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-531759
ABSTRACT
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30 percent (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Protoplastos
/
Ascomicetos
/
Basidiomycota
/
Estabilidad de Enzimas
/
Fusión Génica
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Canadá
/
Grecia
Institución/País de afiliación:
Fellow Alberta Research Council/CA
/
University of Patras/GR
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