Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
Biol. Res
;
42(3): 339-349, 2009. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-531967
ABSTRACT
We had recently reported antibacterial activity in the crude extract of the stem bark of Afzelia africana (Akinpelu et al., 2008). In this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. The aqueous (AQ) and butanol (BL) fractions exhibited appreciable antibacterial activities against the test bacteria. The minimum inhibitory concentrations of the AQ and BL fractions ranged between 0.313 and 2.5 mg/ml, while their minimum bactericidal concentrations varied between 0.625 and 5.0 mg/ml. Also, the AQ fraction killed about 95.8 percent of E. coli cells within 105 min at a concentration of 5 mg/ml, while about 99.1 percent of Bacillus pumilus cells were killed by this fraction at the same concentration and exposure time. A similar trend was observed for the BL fraction. At a concentration of 5 mg/ml, the butanol fraction leaked 9.8 μg/ml of proteins from E. coli cells within 3 h, while the aqueous fraction leaked 6.5 μg/ml of proteins from the same organisms at the same concentration and exposure time. We propose that the stem bark of Afzelia africana is a potential source of bioactive compounds of importance to the pharmaceutical industry.
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LILACS (Américas)
Asunto principal:
Extractos Vegetales
/
Bacterias Gramnegativas
/
Bacterias Grampositivas
/
Fabaceae
/
Antibacterianos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Sudáfrica
Institución/País de afiliación:
University of Fort Hare/ZA
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