Antimicrobial activity of sodium hypochlorite associated with intracanal medication for Candida albicans and Enterococcus faecalis inoculated in root canals

ABSTRACT

OBJECTIVE: The purpose of this study was to evaluate the action of sodium hypochlorite (NaOCl) associated with an intracanal medication against Candida albicans and Enterococcus faecalis inoculated in root canals. MATERIAL AND METHODS: Thirty-six human single-rooted teeth with single root canals were used. The canals were contaminated with C. albicans and E. faecalis for 21 days and were then instrumented with 1 percent NaOCl. The roots were divided into 3 groups (n=12) according to the intracanal medication applied calcium hydroxide paste, 2 percent chlorhexidine (CHX) gel, and 2 percent CHX gel associated with calcium hydroxide. The following collections were made from the root canals a) initial sample (IS) 21 days after contamination (control), b) S1 after instrumentation, c) S2 14 days after intracanal medication placement; S3 7 days after intracanal medication removal. The results were analyzed statistically by the Kruskal-Wallis test at 5 percent significance level. RESULTS AND CONCLUSIONS: Both 1 percent NaOCl irrigation and the intracanal medications were effective in eliminating E. faecalis and C. albicans inoculated in root canals.