Metallo-â-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil
Braz. j. infect. dis
;
13(3): 170-172, June 2009. tab
Artículo
en Inglés
| LILACS
| ID: lil-538515
ABSTRACT
This study determined the prevalence of metallo-â-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Rio Grande do Sul, Brazil (twenty-three from a hospital in Porto Alegre City and eight isolates from a hospital in Vale dos Sinos Region). All strains suggestive of possessing MBLs by phenotypic methods were included in this study. Phenotypic detection of MBLs was carried out simultaneously by using both the MBL Etest® and disk approximation test using 2-mercaptopropionic acid close to a ceftazidime disk. Strains positive were further confirmed using molecular techniques for blaVIM, blaIMP and blaSPM-1. The prevalence of MBLs from samplesof inpatients from the hospital located in Porto Alegre was 30.4 percent and that of inpatients from Vale dos Sinos hospital was only 3.1 percent. Only MBL type SPM-1 was detected in these samples by molecular analysis and all were detected by the Etest® MBL strips. The prevalence of P. aeruginosa that produce MBLs can be markedly different in distinct geographical areas, even among different hospitals in the same area. In our study, the EDTA-based method was the only method able to detect all strains harboring the SPM-1 enzyme.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Pseudomonas aeruginosa
/
Beta-Lactamasas
Tipo de estudio:
Factores de riesgo
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. infect. dis
Asunto de la revista:
Enfermedades Transmisibles
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Exame Laboratory/BR
/
Feevale, Laboratory of Biomedicine/BR
/
Unilab/BR
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