Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
Braz. arch. biol. technol
;
52(6): 1349-1356, Nov.-Dec. 2009. tab, ilus
Artículo
en Inglés
| LILACS
| ID: lil-539101
ABSTRACT
Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3 percent, inoculum volume 7 percent, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
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LILACS (Américas)
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
India
Institución/País de afiliación:
Indian Institute of Technology/IN
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