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Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
Kumari, Annapurna; Mahapatra, Paramita; Banerjee, Rintu.
  • Kumari, Annapurna; Indian Institute of Technology. Agricultural and Food Engineering Department. Microbial Biotechnology and Downstream Processing Laboratory. Kharagpur. IN
  • Mahapatra, Paramita; Indian Institute of Technology. Agricultural and Food Engineering Department. Microbial Biotechnology and Downstream Processing Laboratory. Kharagpur. IN
  • Banerjee, Rintu; Indian Institute of Technology. Agricultural and Food Engineering Department. Microbial Biotechnology and Downstream Processing Laboratory. Kharagpur. IN
Braz. arch. biol. technol ; 52(6): 1349-1356, Nov.-Dec. 2009. tab, ilus
Artículo en Inglés | LILACS | ID: lil-539101
ABSTRACT
Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3 percent, inoculum volume 7 percent, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.

Texto completo: Disponible Índice: LILACS (Américas) Tipo de estudio: Estudio pronóstico Idioma: Inglés Revista: Braz. arch. biol. technol Asunto de la revista: Biologia Año: 2009 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Indian Institute of Technology/IN

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Texto completo: Disponible Índice: LILACS (Américas) Tipo de estudio: Estudio pronóstico Idioma: Inglés Revista: Braz. arch. biol. technol Asunto de la revista: Biologia Año: 2009 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Indian Institute of Technology/IN