Beneficial effects of n-acetyl cysteine on pancreas and kidney following experimental pancreatic ischemia-reperfusion in rats
Clinics
; 65(3): 311-316, 2010. tab, ilus
Article
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| LILACS
| ID: lil-544011
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BR1.1
ABSTRACT
OBJECTIVE:
To evaluate the protective effects of N-acetyl cysteine on the pancreas and kidney after pancreatic ischemia reperfusion injury in a rat model. METHODS AND MATERIALS Pancreatic ischemia reperfusion was performed in Wistar rats for 1 hour. Revascularization was achieved followed by 4 h of reperfusion. A total of 24 animals were divided into four groups Group 1 sham; Group 2 pancreatic ischemia reperfusion without treatment; Group 3 pancreatic ischemia reperfusion plus N-acetyl cysteine intravenously; and Group 4 pancreatic ischemia reperfusion plus N-acetyl cysteine per os. Blood and tissue samples were collected after reperfusion.RESULTS:
There were significant differences in amylase levels between Group 1 (6.11±0.55) and Group 2 (10.30±0.50) [p=0.0002] as well as between Group 2 (10.30±0.50) and Group 4 (7.82±0.38) [p=0.003]; creatinine levels between Group 1 (0.52 ± 0.07) and Group 2 (0.77±0.18) [p=0.035] as well as between Group 2 (0.77±0.18) and Group 3 (0.48±0.13) [p=0.012]; and pancreatic tissue thiobarbituric acid reactive substance levels between Group 1 (1.27±0.96) and Group 2 (2.60±3.01) [p=0.026] as well as between Group 2 (2.60±3.01) and Group 4 (0.52±0.56) [p=0.002]. A decrease in pancreatic tissue GST-á3 gene expression was observed in Group 2 in comparison to Group 1 (p =0.006), and an increase was observed in Groups 3 and 4 when compared to Group 2 (p= 0.025 and p=0.010, respectively).CONCLUSION:
This study provides evidence that N-acetyl cysteine has a beneficial effect on pancreatic ischemia reperfusion injury and renal function in a rat model.Palabras clave
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LILACS
Asunto principal:
Páncreas
/
Acetilcisteína
/
Daño por Reperfusión
/
Riñón
Límite:
Animals
Idioma:
En
Revista:
Clinics
Asunto de la revista:
MEDICINA
Año:
2010
Tipo del documento:
Article