Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
Mem. Inst. Oswaldo Cruz
;
105(2): 132-136, Mar. 2010. ilus
Artículo
en Inglés
| LILACS
| ID: lil-544617
ABSTRACT
In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Rhodnius
/
Glándulas Salivales
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Ezequiel Dias/BR
/
Universidade Federal de Minas Gerais/BR
Similares
MEDLINE
...
LILACS
LIS