Evaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia
Mem. Inst. Oswaldo Cruz
;
105(2): 117-122, Mar. 2010. ilus
Artículo
en Inglés
| LILACS
| ID: lil-544633
ABSTRACT
The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram-positive bacteria belonging to six species and Gram-negative bacteria from 10 species were isolated and identified by culture and detected using broad-range 16S rDNA primers and Gram-specific primers. Data from the three methods all conferred very high sensitivity, specificity, positive and negative predictive values when compared to whole blood culture. The Kappa coefficients of agreement were 0.9819 (buffy coat PCR), 0.9458 (whole blood PCR) and 1.0 (buffy coat culture), which establishes their validity as alternative methods to routine blood culture in detecting bacteraemia. In addition, results showed that there was a direct correlation of WBC counts greater than 12,000 cells per mm³ to the occurrence of bacteraemia as detected by the four methods (p < 0.05).
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Índice:
LILACS (Américas)
Asunto principal:
Bacteriemia
/
Bacterias Gramnegativas
/
Bacterias Grampositivas
/
Leucocitos
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
/
Estudio pronóstico
Límite:
Adolescente
/
Adulto
/
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Pakistán
/
Filipinas
Institución/País de afiliación:
De La Salle University/PH
/
Makati Medical Center/PH
/
Ospital ng Makati/PH
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