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A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
Kumar, Gavish; Shankar, Hari; Bisht, Deepa; Sharma, Prashant; Singhal, Neelja; Katoch, V. M; Joshi, Beenu.
  • Kumar, Gavish; National JALMA Institute for Leprosy and Other Mycobacterial Diseases. Department of Immunology. Agra. IN
  • Shankar, Hari; National JALMA Institute for Leprosy and Other Mycobacterial Diseases. Department of Immunology. Agra. IN
  • Bisht, Deepa; National JALMA Institute for Leprosy and Other Mycobacterial Diseases. Department of Biochemistry. Agra. IN
  • Sharma, Prashant; National JALMA Institute for Leprosy and Other Mycobacterial Diseases. Department of Biochemistry. Agra. IN
  • Singhal, Neelja; National JALMA Institute for Leprosy and Other Mycobacterial Diseases. Department of Biochemistry. Agra. IN
  • Katoch, V. M; Indian Council of Medical Research. Department of Health Research. New Delhi. IN
  • Joshi, Beenu; National JALMA Institute for Leprosy and Other Mycobacterial Diseases. Department of Immunology. Agra. IN
Braz. j. microbiol ; 41(2): 295-299, Apr.-June 2010. ilus
Artículo en Inglés | LILACS | ID: lil-545332
ABSTRACT
Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Cromatografía en Gel / Medios de Cultivo / Lípidos / Mycobacterium tuberculosis Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2010 Tipo del documento: Artículo / Documento de proyecto País de afiliación: India Institución/País de afiliación: Indian Council of Medical Research/IN / National JALMA Institute for Leprosy and Other Mycobacterial Diseases/IN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Cromatografía en Gel / Medios de Cultivo / Lípidos / Mycobacterium tuberculosis Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2010 Tipo del documento: Artículo / Documento de proyecto País de afiliación: India Institución/País de afiliación: Indian Council of Medical Research/IN / National JALMA Institute for Leprosy and Other Mycobacterial Diseases/IN