Detection of brucella ovis in ovine from Paraíba state, in the northeast region of Brazil
Braz. j. microbiol
;
41(2): 365-367, Apr.-June 2010. ilus
Artículo
en Inglés
| LILACS
| ID: lil-545343
ABSTRACT
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5 percent) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Técnicas In Vitro
/
Brucelosis Bovina
/
Reacción en Cadena de la Polimerasa
/
Brucella ovis
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Campina Grande/BR
/
Universidade de São Paulo/BR
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