Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
Mem. Inst. Oswaldo Cruz
;
105(3): 331-335, May 2010. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-547306
ABSTRACT
The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segments was performed using two regions of cagA 349 bp were amplified with the F1/B1 primers and the remaining 335 bp were amplified with the B7629/B7628 primers. The VA1-F/VA1-R set of primers was used to amplify the 259-bp (s1) or 286-bp (s2) product and the VAG-R/VAG-F set of primers was used to amplify the 567-bp (m1) or 642-bp (m2) regions of vacA. cagA was detected in 87 percent of the antral samples from Cuban patients and 80.3 percent of those from Venezuelan patients. All possible combinations of vacA regions were found, with the exception of s2/m1. The predominant combination found in both countries was s1/m1. The percentage of cagA+ strains was increased by the use of a second set of primers and a greater number of strains was amplified with the B7629/B7628 primers in the Cuban patients (p = 0.0001). There was no significant difference between the presence of the allelic variants of vacA and cagA in both populations. The predominant genotype was cagA+/s1m1 in both countries. The results support the necessary investigation of isolates circulating among the human population in each region.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteínas Bacterianas
/
Helicobacter pylori
/
Infecciones por Helicobacter
/
Gastritis
/
Antígenos Bacterianos
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Sur
/
Caribe
/
Cuba
/
Venezuela
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Cuba
/
Francia
/
México
/
Venezuela
Institución/País de afiliación:
INSERM/FR
/
Instituto Mexicano del Seguro Social/MX
/
United Nations Framework Convention on Climate Change de La Habana/CU
/
Universidad Central de Venezuela/VE
Similares
MEDLINE
...
LILACS
LIS