Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
Mem. Inst. Oswaldo Cruz
;
105(3): 353-355, May 2010. ilus
Artículo
en Inglés
| LILACS
| ID: lil-547309
ABSTRACT
The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Microbiología del Agua
/
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Leptospira
Tipo de estudio:
Estudio diagnóstico
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
PAHO/BR
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