Isolation of high quality DNA: a protocol combining rennet and glass milk
Electron. j. biotechnol
;
12(2): 11-12, Apr. 2009. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-551372
ABSTRACT
High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first protocol utilizes the enzyme chymosin, normally used as rennet in cheese production and which is easily obtained on the commercial market. The second protocol uses rennet DNA extraction protocol combined with the DNA binding capacity of glass powder (glass milk), which can easily be home made. The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
ADN
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Quimosina
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Leche
Tipo de estudio:
Guía de Práctica Clínica
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Santa Maria/BR
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