Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
Genet. mol. biol
;
33(3): 460-462, 2010. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-555830
ABSTRACT
Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through proteinprotein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC samples studied. A co-localization of GSTP1 and JNK was also demonstrated in the 5637 TCC cell line by means of confocal microscopy. Protein-protein interactions, together with co-localization between GSTP1 and JNK provide evidence that GSTP1 most probably inhibits apoptosis in TCC cells by non-covalent binding to JNK.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Neoplasias de la Vejiga Urinaria
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Glutatión Transferasa
Límite:
Humanos
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
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