Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction
J. appl. oral sci
;
18(4): 426-431, July-Aug. 2010. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-557116
ABSTRACT
OBJECTIVE:
The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR).METHODOLOGY:
Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine prevalence rates, which were then compared using a chi-square test. Significance level was set at p<0.05. Mean and standard deviation values were used to evaluate variations in prevalence according to site.RESULTS:
The prevalence of S. mutans was 70 percent in saliva; 60 percent in samples collected from the tongue dorsum; 50 percent in samples collected from the buccal mucosa; 56.5 percent in the supragingival plaque; and 53.5 percent in the subgingival plaque. The prevalence of E. faecalis ranged from 3.5 percent to 13.5 percent in all intraoral microenvironment. The highest prevalence of P. gingivalis was found in subgingival plaque (53.5 percent), and of P. intermedia in supragingival plaque (33.5 percent), subgingival plaque (30 percent) and tongue dorsum (33.5 percent). The prevalence of bacteria did not vary significantly among the intraoral sites.CONCLUSIONS:
All studied bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to be an adequate method for epidemiological studies.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Bacteroidaceae
/
Reacción en Cadena de la Polimerasa
/
Periodontitis Crónica
/
Lactobacillales
Tipo de estudio:
Estudio diagnóstico
/
Factores de riesgo
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
J. appl. oral sci
Asunto de la revista:
Odontología
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Goiás/BR
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