Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis
J. venom. anim. toxins incl. trop. dis
;
16(3): 414-420, 2010. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-557169
ABSTRACT
Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100 percent whereas ELISA was 99 percent. Sensitivities were 97.77, 93.33 and 91.11 percent respectively for IFAT, ELISA and PCR.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Leishmaniasis Visceral
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
Toxicología
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual Paulista/BR
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