Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
Mem. Inst. Oswaldo Cruz
;
105(5): 687-691, Aug. 2010. graf
Artículo
en Inglés
| LILACS
| ID: lil-557231
ABSTRACT
The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Trypanosoma cruzi
/
Proteínas Protozoarias
/
Glicoproteínas Variantes de Superficie de Trypanosoma
/
Vacunas Antiprotozoos
/
Enfermedad de Chagas
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Estados Unidos
Institución/País de afiliación:
Saint Louis University/US
/
Universidade Federal de São Paulo/BR
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