Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients
Mem. Inst. Oswaldo Cruz
;
105(5): 722-728, Aug. 2010. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-557238
ABSTRACT
To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2 percent, 47.4 percent; 95 percent, 93.7 percent; 40 percent, 98 percent and 28.4 percent, 97.1 percent, respectively). However, while grey zone was 12.7 percent and 6 percent, respectively, lowering sensitivity but maintains high specificity (> 95 percent). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tuberculosis Meníngea
/
Inmunoglobulina A
/
Inmunoglobulina G
/
Anticuerpos Antibacterianos
/
Mycobacterium tuberculosis
/
Antígenos Bacterianos
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Límite:
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
/
Alemania
Institución/País de afiliación:
Lionex Diagnostics and Therapeutics/DE
/
Universidade Federal do Rio de Janeiro/BR
Similares
MEDLINE
...
LILACS
LIS