Normalized embryoid cDNA library of oil palm (Elaeis guineensis) / Biblioteca ADNc normalizada de embrioides en aceite de palma (Elaeis guineensis)
Electron. j. biotechnol
;
13(1): 10-11, Jan. 2010. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-559593
ABSTRACT
A normalized embryoid cDNA library (EON) was constructed based on reassociation kinetics reaction. Results from dot blot hybridization and sequencing of EON cDNA clones clearly indicated that the normalization process reduced the frequency of high abundance transcripts and increased the frequency of low abundance gene transcripts. A total of 553 non-redundant expressed sequence tags (ESTs) were identified, 325 of these were not observed in the standard oil palm cDNA libraries sequenced previously. A total of 10 EON cDNA clones were chosen for expression profiling across samples from different stages of the tissue culture process. Two of the genes exhibited promising expression patterns for predicting the embryogenic potential in callus. Some of these genes were also differentially expressed in the various tissues of oil palm. This study showed that normalization of the existing embryoid library improved the chances of identifying transcripts not captured in the standard libraries, some of which could be associated with embryogenesis. This collection of ESTs is particularly well suited for use as candidate genes for development of an oil palm DNA chip, which can be used to obtain a more comprehensive view of the molecular mechanism associated with oil palm tissue culture.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ARN Mensajero
/
Aceite de Palma
/
ADN Complementario
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
China
/
Malasia
Institución/País de afiliación:
ACGT Sdn Bhd/MY
/
Academia Sinica/TW
/
Malaysian Palm Oil Board/MY
/
Myagri Associates Sdn Bhd/MY
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