IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
Mem. Inst. Oswaldo Cruz
;
105(6): 757-761, Sept. 2010. ilus
Artículo
en Inglés
| LILACS
| ID: lil-560659
ABSTRACT
The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5 percent and a specificity of 97.4 percent. IgM-WB showed a sensitivity of 54.8 percent and a specificity of 94.7 percent. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5 percent. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Toxoplasma
/
Inmunoglobulina G
/
Inmunoglobulina M
/
Anticuerpos Antiprotozoarios
/
Toxoplasmosis Congénita
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Límite:
Humanos
/
Recién Nacido
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
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