Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the beta-glucosidase gene
Electron. j. biotechnol
;
13(2): 5-6, Mar. 2010. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-567083
ABSTRACT
In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing the beta-glucosidase gene from Humicola grisea was used for ethanol production from three different cellulosic sources by simultaneous saccharification and fermentation. Initially, a enzymatic pre-hydrolysis step was done with a solidliquid ratio of 14, and an enzymatic load of 25 filter paper activity (FPU).g-1 of cellulosic substrate. Using sugarcane bagasse pretreated cellulignin, crystalline cellulose and carboxymethyl cellulose, 51.7 g L-1, 41.7 g L-1 and 13.8 g L-1 of ethanol was obtained, respectively, at the end of 55 hrs of fermentation. The highest ethanol productivity (0.94 g L-1 hrs-1) was achieved using sugarcane bagasse pretreated cellulignin. The use of a recombinant S. cerevisiae led to extremely low glucose concentrations when compared to other works reported in literature.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Saccharomyces cerevisiae
/
Celulosa
/
Beta-Glucosidasa
/
Etanol
/
Lignina
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal do Rio de Janeiro/BR
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