Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates
Braz. j. microbiol
;
42(1): 225-232, Jan.-Mar. 2011. tab
Artículo
en Inglés
| LILACS
| ID: lil-571393
ABSTRACT
Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6 percent), L. jensenii (25 percent) and L. gasseri (20.6 percent) were the most frequent species. However, using the biochemical technique, the most frequent species were L. acidophilus (34.8 percent), L. crispatus (27.2 percent) and L. fermentum (13 percent). Although L. acidophilus was the most frequent specie found by biochemical tests, no strain of this microorganism was detected by PCR. Agreement between the methods was low for identification of all the most common species. Although rates of L. crispatus detected were similar using both methods (32.6 percent and 27.2 percent), agreement between them was relatively low (kappa = 0.52). Conclusions:
Our results confirmed the limitation of the biochemical method and the applicability of a previously published molecular method (Multiplex PCR) for the identification of lactobacilli in the vaginal tract, focusing on further necessity of its improvement for also targeting L. vaginalis and L. iners.
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LILACS (Américas)
Asunto principal:
Fenotipo
/
Infecciones Urinarias
/
Técnicas In Vitro
/
Carbohidratos
/
Reacción en Cadena de la Polimerasa
/
Vaginosis Bacteriana
/
Infecciones por Bacterias Grampositivas
/
Ecosistema
/
Fermentación
/
Lactobacillus
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
Límite:
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual Paulista/BR
/
Universidade Estadual de Campinas/BR
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