Human T-Lymphotropic virus (HTLV) type I in vivo integration in oral keratinocytes
Braz. j. microbiol
;
42(1): 310-320, Jan.-Mar. 2011. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-571405
ABSTRACT
Although the infection of HTLV-1 to cell components of the mouth have been previously reported, there was not until this report, a detailed study to show the characteristics of such infection. From 14 Tropical Spastic Paraparesis/ HTLV-1-Associated Myelopathy (HAM/TSP) patients and 11 asymptomatic carrier individuals (AC) coming from HTLV-1 endemic areas of southwest Pacific of Colombia, infected oral mucosa cells were primary cultured during five days. These cell cultures were immunophenotyped by dual color fluorescence cell assortment using different lymphocyte CD markers and also were immunohistochemically processed using a polyclonal anti-keratin antibody. Five days old primary cultures were characterized as oral keratinocytes, whose phenotype was CD3- /CD4-/CD8-/CD19-/CD14-/CD45-/A575-keratin+. From DNA extracted of primary cultures LTR, pol, env and tax HTLV-1 proviral DNA regions were differentially amplified by PCR showing proviral integration. Using poly A+ RNA obtained of these primary cultures, we amplify by RT-PCR cDNA of tax and pol in 57.14 percent (8/14) HAM/TSP patients and 27.28 percent (3/11) AC. Tax and pol poly A+ RNA were expressed only in those sIgA positive subjects. Our results showed that proviral integration and viral gene expression in oral keratinocytes are associated with a HTLV-1 specific local mucosal immune response only in those HTLV-1 infected individuals with detectable levels of sIgA in their oral fluids. Altogether the results gave strong evidence that oral mucosa infection would be parte of the systemic spreading of HTLV-1 infection.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones Tumorales por Virus
/
Técnicas In Vitro
/
Inmunohistoquímica
/
Anticuerpos Antideltaretrovirus
/
Queratinocitos
/
Reacción en Cadena de la Polimerasa
/
Inmunofenotipificación
/
VIH
/
Virus de la Reticuloendoteliosis
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Colombia
Institución/País de afiliación:
University of Valle/CO
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