Human sulfatase transiently and functionally active expressed in E. coli K12
Electron. j. biotechnol
;
13(3): 5-6, May 2010. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-577100
ABSTRACT
The recombinant human iduronate 2-sulfate sulfatase (hrIDS) was transiently and functionally active expressed in E. coli K12. The enzyme activity (crude extract) at 100 ml and 400 ml oscillated between 0.25 and 10.58 nmol h-1 mg-1. The wide Western-blot peptide profile suggest that hrIDS is proteolitically processed randomly which agrees with the ultrafiltration assay in which the hrIDS activity was found in all fractions (<30kDa, 30-100kDa and >100kDa). No glycation sites were found by computer analysis of the hIDS sequence; discarding the possibility of marks for glycation and proteolytic processing.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteínas Recombinantes
/
Iduronato Sulfatasa
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Colombia
Institución/País de afiliación:
Pontificia Universidad Javeriana/CO
/
Universidad del Quindío/CO
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