Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
Electron. j. biotechnol
;
13(4): 6-7, July 2010. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-577111
ABSTRACT
Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, including six fermentation medium components and two cell adsorption conditions. Among the variables screened, soybean meal hydrolysate (SMH) and MgSO4A7H2O in the fermentation medium had significant effects on JAA production. In the second step, the concentrations of SMH and MgSO4A7H2O were further optimized using central composite designs and response surface analysis. The optimized concentration of SMH and MgSO4À7H2O was 24 percent (v/v) and 0.38 percent (w/v), respectively, which increased the production of JAA in a shake flask system by 41 percent relative to optimization of a single variable component of the culture medium.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Péptidos
/
Bacillus subtilis
/
Madera
/
Fermentación
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2010
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
China
Institución/País de afiliación:
Chengdu Institute of Biology Chinese Academy of Sciences/CN
/
Graduate University of Chinese Academy of Sciences/CN
Similares
MEDLINE
...
LILACS
LIS