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Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil
Wollheim, Claudia; Guerra, Ivani Maria F; Conte, Vania D; Hoffman, Sheila P; Schreiner, Fernando J; Delamare, Ana Paula L; Barth, Afonso L; Echeverrigaray, Sérgio; Costa, Sérgio Olavo P da.
  • Wollheim, Claudia; Universidade de Caxias do Sul. Medical Microbiology Laboratory. BR
  • Guerra, Ivani Maria F; Universidade de Caxias do Sul. Medical Microbiology Laboratory. BR
  • Conte, Vania D; Universidade de Caxias do Sul. Medical Microbiology Laboratory. BR
  • Hoffman, Sheila P; Universidade de Caxias do Sul. Medical Microbiology Laboratory. BR
  • Schreiner, Fernando J; Universidade de Caxias do Sul. Medical Microbiology Laboratory. BR
  • Delamare, Ana Paula L; Universidade de Caxias do Sul. Instituto de Biotecnologia. BR
  • Barth, Afonso L; Hospital de Clínicas de Porto Alegre. BR
  • Echeverrigaray, Sérgio; Universidade de Caxias do Sul. Instituto de Biotecnologia. BR
  • Costa, Sérgio Olavo P da; Universidade de Caxias do Sul. Instituto de Biotecnologia. BR
Braz. j. infect. dis ; 15(2): 138-143, Mar.-Apr. 2011. ilus, tab
Artículo en Inglés | LILACS | ID: lil-582416
ABSTRACT

OBJECTIVES:

To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections.

METHODS:

The study involved 354 nosocomial infections samples and 992 community infections samples, obtained between 2003 and 2006 at Caxias do Sul, RS. The detection of ESBL was performed by the disk-diffusion test. Presence of blaCTX-M, blaSHV and blaTEM β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis analysis.

RESULTS:

Higher frequency of ESBL-producing isolates were detected among nosocomial samples of E. coli (6.7 percent) and Klebsiella (43.7 percent), than those obtained from community infections (0.4 percent and 2.6 percent). blaTEM and blaCTX were the most prevalent ESBL gene families in both E. coli and Klebsiella isolates. Different pulsotypes were obtained among ESBL-producing E. coli and 11 clones for Klebsiella spp., which occurred over the years and in different hospital wards. Among ESBL-producing K. pneumoniae, 74.3 percent transferred ESBL genes by conjugation and exhibited concomitant decreased aminoglycosides susceptibility.

CONCLUSION:

ESBL-producing E. coli, and especially K. pneumoniae are essentially a nosocomial problem, and their dissemination to the community is relatively limited. The great genetic variability observed among ESBL-producing bacteria indicates polyclonal spread and high transference of ESBL genes between bacteria in the hospital environment. This information is of paramount importance for nosocomial infection control.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Beta-Lactamasas / Infecciones por Klebsiella / Infección Hospitalaria / Escherichia coli / Infecciones por Escherichia coli / Klebsiella Tipo de estudio: Estudio de prevalencia / Factores de riesgo Límite: Adulto / Femenino / Humanos / Masculino País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: Braz. j. infect. dis Asunto de la revista: Enfermedades Transmisibles Año: 2011 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Hospital de Clínicas de Porto Alegre/BR / Universidade de Caxias do Sul/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Beta-Lactamasas / Infecciones por Klebsiella / Infección Hospitalaria / Escherichia coli / Infecciones por Escherichia coli / Klebsiella Tipo de estudio: Estudio de prevalencia / Factores de riesgo Límite: Adulto / Femenino / Humanos / Masculino País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: Braz. j. infect. dis Asunto de la revista: Enfermedades Transmisibles Año: 2011 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Hospital de Clínicas de Porto Alegre/BR / Universidade de Caxias do Sul/BR