Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
Mem. Inst. Oswaldo Cruz
;
106(2): 200-206, Mar. 2011. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-583946
ABSTRACT
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100 percent sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100 percent sensitivity at a dilution of 140, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Inmunoglobulina G
/
Inmunoglobulina M
/
Anticuerpos Antihelmínticos
/
Toxocariasis
/
Toxocara canis
/
Antígenos Helmínticos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Hospital Santa Casa de Belo Horizonte/BR
/
Universidade Federal de Minas Gerais/BR
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