Morphological and molecular description of Blastocrithidia cyrtomeni sp. nov. (Kinetoplastea: Trypanosomatidae) associated with Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) from Colombia
Mem. Inst. Oswaldo Cruz
;
106(3): 301-307, May 2011. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-589038
ABSTRACT
A new trypanosomatid species, Blastocrithidia cyrtomeni, is herein described using morphological and molecular data. It was found parasitising the alimentary tract of the insect host Cyrtomenus bergi, a polyphagous pest. The morphology of B. cyrtomeni was investigated using light and transmission microscopy and molecular phylogeny was inferred from the sequences of spliced leader RNA (SL rRNA) - 5S rRNA gene repeats and the 18S small subunit (SSU) rRNA gene. Epimastigotes of variable size with straphanger cysts adhering to the middle of the flagellum were observed in the intestinal tract, hemolymph and Malpighian tubules. Kinetoplasts were always observed anterior to the nucleus. The ultrastructure of longitudinal sections of epimastigotes showed the flagellum arising laterally from a relatively shallow flagellar pocket near the kinetoplast. SL RNA and 5S rRNA gene repeats were positive in all cases, producing a 0.8-kb band. The amplicons were 797-803 bp long with > 98.5 percent identity, indicating that they originated from the same organism. According to the sequence analysis of the SL-5S rRNA gene repeats and the 18S SSU rRNA gene, B. cyrtomeni is different from all other known species or isolates of Trypanosomatidae. Both analyses indicate that among known species, it is most closely related to Blastocrithidia triatomae.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ADN Protozoario
/
ARN Protozoario
/
Trypanosomatina
/
ARN Lider Empalmado
/
Hemípteros
Tipo de estudio:
Factores de riesgo
Límite:
Animales
País/Región como asunto:
America del Sur
/
Colombia
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Colombia
/
Estados Unidos
Institución/País de afiliación:
International Center of Tropical Agriculture/CO
/
Universidad Nacional de Colombia/CO
/
Universidad del Cauca/CO
/
Universidad del Valle/CO
/
University of California/US
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