Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum
Electron. j. biotechnol
;
14(1): 12-13, Jan. 2011. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-591930
ABSTRACT
As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools geNorm and NormFinder. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). NormFinder as well as geNorm identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes differed depending on the algorithm. According to NormFinder COG complex component displayed the most stable expression whereas geNorm indicated V-ATPase G subunit 1 and a putative ABC transporter ATPase to be the most reliable reference genes. Hence, we concluded to use a normalization factor calculated from the four reference genes V-ATPase G subunit 1, ABC transporter ATPase, Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4) and COG complex component for normalization of qRT-PCR in cell cultures of Cyclamen persicum.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Cyclamen
/
Desarrollo Embrionario
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Alemania
Institución/País de afiliación:
Leibniz-Institute of Vegetable and Ornamental Crops/DE
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