Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture
Biocell
;
33(3): 179-186, Dec. 2009. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-595022
ABSTRACT
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 microM thidiazuron and 4.44 microM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 microM alpha-naphthalenacetic acid, 13.95 microM kinetin and 13.32 microM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 microM alpha-naphthalenacetic acid.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Reguladores del Crecimiento de las Plantas
/
Células Cultivadas
/
Organogénesis
/
Fabaceae
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional del Nordeste/AR
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