Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production
Biocell
;
34(1): 1-6, Apr. 2010. tab, ilus
Artículo
en Inglés
| LILACS
| ID: lil-595044
ABSTRACT
Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 microM). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem internode was the highest producer of baccatin III using 1 microM elicit or, sampling at 10 days.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Paclitaxel
/
Taxus
/
Ciclopentanos
/
Proliferación Celular
/
Alcaloides
/
Acetatos
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
México
Institución/País de afiliación:
Instituto Tecnológico de Veracruz/MX
/
Instituto de Ecología/MX
/
Universidad Veracruzana/MX
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