Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants
Biocell
;
34(1): 7-13, Apr. 2010. ilus
Artículo
en Inglés
| LILACS
| ID: lil-595045
ABSTRACT
Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 microM thidiazuron or 0.45 microM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was 28%, and subcultured in groups the conversion declined to 6.8%. Fused embryos subcultured in groups had only a 2.1% conversion and produced plants with fused stems. None conversion rate in the others classes was associated to poorly developed shoot and root meristematic areas or with their absence. The converted plants were acclimatized and transferred, in a mist, to soil, with an independent of the class 95% survival rate.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Brotes de la Planta
/
Meristema
/
Melia azedarach
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional del Nordeste/AR
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