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Effectiveness of 2 percent peracetic acid for the disinfection of gutta-percha cones
Salvia, Ana Carolina Rodrigues Danzi; Teodoro, Guilherme Rodrigues; Balducci, Ivan; Koga-Ito, Cristiane Yumi; Oliveira, Simone Helena Gonçalves de.
  • Salvia, Ana Carolina Rodrigues Danzi; Univ. Estadual Paulista. São José dos Campos Dental School. Department of Biosciences and Oral Diagnosis. São José dos Campos. BR
  • Teodoro, Guilherme Rodrigues; Univ. Estadual Paulista. São José dos Campos Dental School. Department of Biosciences and Oral Diagnosis. São José dos Campos. BR
  • Balducci, Ivan; Univ. Estadual Paulista. São José dos Campos Dental School. Department of Social Dentistry and Pediatric Dentistry. São José dos Campos. BR
  • Koga-Ito, Cristiane Yumi; Univ. Estadual Paulista. São José dos Campos Dental School. Department of Biosciences and Oral Diagnosis. São José dos Campos. BR
  • Oliveira, Simone Helena Gonçalves de; Univ. Estadual Paulista. São José dos Campos Dental School. Department of Biosciences and Oral Diagnosis. São José dos Campos. BR
Braz. oral res ; 25(1): 23-27, Jan.-Feb. 2011. tab
Artículo en Inglés | LILACS | ID: lil-595835
ABSTRACT
The aim of this study was to evaluate the effectiveness of 2 percent peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10 percent sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100 percent of the microbial inocula were eliminated. It was concluded that the 2 percent peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Ácido Peracético / Materiales de Obturación del Conducto Radicular / Desinfectantes / Gutapercha Límite: Humanos Idioma: Inglés Revista: Braz. oral res Asunto de la revista: Odontología Año: 2011 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Univ. Estadual Paulista/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Ácido Peracético / Materiales de Obturación del Conducto Radicular / Desinfectantes / Gutapercha Límite: Humanos Idioma: Inglés Revista: Braz. oral res Asunto de la revista: Odontología Año: 2011 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Univ. Estadual Paulista/BR