Assessment of Leishmania major and Leishmania braziliensis promastigote viability after photodynamic treatment with aluminum phthalocyanine tetrasulfonate (AlPcS4)
J. venom. anim. toxins incl. trop. dis
;
17(3): 300-307, 2011. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-597229
ABSTRACT
Cutaneous leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania, which is transmitted through the bite of hematophagous insects of the genus Lutzomyia. This study aimed at testing in vitro the phototoxic effect of aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability of Leishmania major and Leishmania braziliensis. Stationary phase promastigote forms were treated with AlPcS4 at 1.0 µM and 10.0 µM and incubated for one hour. Then 659 nm laser was applied at 5 and 10 J/cm². Parasite viability was determined by differential count using the trypan blue dye exclusion method and by monitoring growth curves for nine days. Trypan blue exclusion assay showed a significant reduction of viable parasites compared to controls, L. major seemed more sensitive to the toxic effects of AlPcS4 in the dark. The most effective photodynamic therapy (PDT) was obtained with AlPcS4 at 10.0 µM and 10 J/cm² whereas L. braziliensis showed the highest mortality rate after treatment.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Fotoquimioterapia
/
Leishmania braziliensis
/
Leishmaniasis Cutánea
/
Fármacos Fotosensibilizantes
/
Leishmania major
/
Antiparasitarios
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
Toxicología
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Vale do Paraíba University/BR
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