Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile / Detection of genes associated with drug resistance in Mycobacterium tuberculosis strains isolated in Chile
Rev. méd. Chile
;
139(4): 467-473, abr. 2011. ilus
Artículo
en Español
| LILACS
| ID: lil-597642
ABSTRACT
Background:
The incidence of acquired resistance to antituberculous drugs of Mycobacterium tuberculosis in Chile is approximately 23 percent.Aim:
To analyze the mutations associated with drug resistance in drug resistant strains of Mycobacterium tuberculosis. Material andMethods:
In 28 drug resistant Mycobacterium tuberculosis strains isolated in Chile, genes leading to drug resistance were studied. DNA was amplifed by polymerase chain reaction (PCR) and sequencing was carried out using the ABI PRISM big dye terminator cycle sequencing ready reaction kit.Results:
In rifampicin-resistant strains, the mutations in rpoβ gene were in the codons S531W/L (56 percent), D516Y (16 percent) and D516V (16 percent). The predominant mutation in katG gene was in the codon S315L (73 percent) in isoniazid-resistant strains. The mutation S95T was found in the 71 percent of ciprofoxacin resistant strains. Only one ethambutol resistant strain had the M306I mutation. Three unreported mutations in katG were identifed.Conclusions:
Drug resistance associated mutations of Mycobacterium tuberculosis isolated in Chile were similar to those reported abroad.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Farmacorresistencia Bacteriana Múltiple
/
Genes Bacterianos
/
Mutación
/
Mycobacterium tuberculosis
/
Antituberculosos
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Factores de riesgo
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Chile
Idioma:
Español
Revista:
Rev. méd. Chile
Asunto de la revista:
Medicina
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Chile
Institución/País de afiliación:
Instituto de Salud Pública de Chile/CL
Similares
MEDLINE
...
LILACS
LIS