Effects of diabetes on myocardial capillary density and serum angiogenesis biomarkers in male rats
Clinics
;
66(8): 1419-1424, 2011. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-598398
ABSTRACT
INTRODUCTION:
Cardiovascular disease is one of the main causes of mortality and morbidity in diabetic patients. This study evaluated the effects of diabetes on myocardial capillary density and several serum angiogenic factors including nitric oxide, vascular endothelial growth factor, and soluble vascular endothelial growth factor receptors.METHODS:
Twelve male rats were divided into two groups control and diabetic (n = 6 each). Diabetes was induced with a single dose of streptozotocin (50 mg/kg). After 21 days, capillary density in the myocardial tissue was evaluated using immunohistochemical staining and is reported as capillaries per mm². Blood samples were collected before and after the induction of diabetes.RESULTS:
In the diabetic group, serum nitric oxide and soluble vascular endothelial growth factor receptor 2 concentrations were lower than the levels in the control group, while the level of soluble vascular endothelial growth factor receptor 1 was significantly higher. There was no significant change in the serum vascular endothelial growth factor concentration between the diabetic and control groups; however, the ratio of vascular endothelial growth factor to vascular endothelial growth factor receptor 1 was significantly lower in the diabetic animals. The myocardial capillary density was also lower in the diabetic group compared with the control group (1549 ± 161 vs. 2156 ± 202/mm², respectively).CONCLUSION:
Reduced serum nitric oxide and vascular endothelial growth factor receptor 2 levels, increased serum vascular endothelial growth factor receptor 1 levels and a lower vascular endothelial growth factor to vascular endothelial growth factor receptor 1 ratio may be responsible for the decreased myocardial capillary density in diabetic rats.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Capilares
/
Receptores de Factores de Crecimiento Endotelial Vascular
/
Factores de Crecimiento Endotelial Vascular
/
Diabetes Mellitus Experimental
/
Miocardio
/
Neovascularización Patológica
/
Óxido Nítrico
Límite:
Animales
Idioma:
Inglés
Revista:
Clinics
Asunto de la revista:
Medicina
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Irán
Institución/País de afiliación:
Isfahan University of Medical Sciences/IR
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