Kinetic properties of poly (2'-0-methycytidylic acid)- directed reverse transcriptase reaction
Braz. j. med. biol. res
;
21(3): 445-8, Mar. 1988. tab
Artículo
en Inglés
| LILACS
| ID: lil-60214
RESUMO
Poly (2'-O-methylcytidylic acid) is recognized as a template in reactions catalyzed by RNA-dependent DNA polymerases in the presence of Mn2+ as divalent cation. We report that kinetic data obtained for dGTP and template under optimal experimental conditions in the reaction catalyzed by reverse transcriptase showed some similarities between the poly (2'-O-methylcytidylic acid)Mn2+ and polyribocytidylic acid/Mg2+ systems. The reaction was inhibited by the action of N-ethylmaleimide and novobiocin, and to a lesser extent by ethidium bromide and tetramethyl ethidium bromide
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LILACS (Américas)
Asunto principal:
Poli C
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ADN Polimerasa Dirigida por ARN
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Guanosina Trifosfato
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Virus de la Mieloblastosis Aviar
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1988
Tipo del documento:
Artículo
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