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A preferable approach to clone hLIF cDNA from the genomic DNA
Cui, Yihong; Zhu, Guangqin; Chen, Qiuju; Wang, Yunfei; Yang, Mingming; Song, Yuxuan; Wang, Jiangang; Cao, Binyun.
  • Cui, Yihong; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Zhu, Guangqin; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Chen, Qiuju; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Wang, Yunfei; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Yang, Mingming; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Song, Yuxuan; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Wang, Jiangang; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Cao, Binyun; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Electron. j. biotechnol ; 14(3): 12-12, May 2011. ilus, tab
Artículo en Inglés | LILACS | ID: lil-602989
ABSTRACT
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: ADN Complementario / Factor Inhibidor de Leucemia / Mucosa Bucal Límite: Humanos Idioma: Inglés Revista: Electron. j. biotechnol Asunto de la revista: Biotecnologia Año: 2011 Tipo del documento: Artículo / Documento de proyecto País de afiliación: China Institución/País de afiliación: Northwest Agriculture and Forestry University/CN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: ADN Complementario / Factor Inhibidor de Leucemia / Mucosa Bucal Límite: Humanos Idioma: Inglés Revista: Electron. j. biotechnol Asunto de la revista: Biotecnologia Año: 2011 Tipo del documento: Artículo / Documento de proyecto País de afiliación: China Institución/País de afiliación: Northwest Agriculture and Forestry University/CN