Characterization of free nitrogen fixing bacteria of the genus Azotobacter in organic vegetable-grown Colombian soils
Braz. j. microbiol
;
42(3): 846-858, July-Sept. 2011. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-607513
ABSTRACT
With the purpose of isolating and characterizing free nitrogen fixing bacteria (FNFB) of the genus Azotobacter, soil samples were collected randomly from different vegetable organic cultures with neutral pH in different zones of Boyacá-Colombia. Isolations were done in selective free nitrogen Ashby-Sucrose agar obtaining a recovery of 40 percent. Twenty four isolates were evaluated for colony and cellular morphology, pigment production and metabolic activities. Molecular characterization was carried out using amplified ribosomal DNA restriction analysis (ARDRA). After digestion of 16S rDNA Y1-Y3 PCR products (1487pb) with AluI, HpaII and RsaI endonucleases, a polymorphism of 16 percent was obtained. Cluster analysis showed three main groups based on DNA fingerprints. Comparison between ribotypes generated by isolates and in silico restriction of 16S rDNA partial sequences with same restriction enzymes was done with Gen Workbench v.2.2.4 software. Nevertheless, Y1-Y2 PCR products were analysed using BLASTn. Isolate C5T from tomato (Lycopersicon esculentum) grown soils presented the same in silico restriction patterns with A. chroococcum (AY353708) and 99 percent of similarity with the same sequence. Isolate C5CO from cauliflower (Brassica oleracea var. botrytis) grown soils showed black pigmentation in Ashby-Benzoate agar and high similarity (91 percent) with A. nigricans (AB175651) sequence. In this work we demonstrated the utility of molecular techniques and bioinformatics tools as a support to conventional techniques in characterization of the genus Azotobacter from vegetable-grown soils.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Ribosomas
/
Microbiología del Suelo
/
Técnicas In Vitro
/
ADN Ribosómico
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Agar
/
Genética Microbiana
/
Fijación del Nitrógeno
País/Región como asunto:
America del Sur
/
Colombia
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Colombia
/
Alemania
Institución/País de afiliación:
Pontificia Universidad Javeriana/CO
/
University of Bonn/DE
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