Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells
Braz. oral res
;
25(6): 500-505, Nov.-Dec. 2011. ilus
Artículo
en Inglés
| LILACS
| ID: lil-608017
ABSTRACT
Mononuclear cells play an important role in the modulation of healing. The characteristics of implant surface topography may alter the production of signaling molecules such as cytokines. The aim of this in vitro study was to evaluate the effects of commercially available titanium surface treatments on both cell viability and the secretion of the antagonist cytokines, IL1β and TGFβ1. Human mononuclear cells were cultured on 10 mm diameter commercially pure titanium (cpTi) disks that were prepared using a turning procedure (control=machined surface) and either acid etched or bio-anodized for 1-7 days. Adhered cells were investigated with respect to cell viability using an MTT assay, and cytokine production was verified using an ELISA assay. The results indicate that surface characteristics did not alter the cell viability at days 1 and 4, although the machined surface presented the highest absorbance values at day 7 (p = 0.0084). Cell viability was reduced throughout the time course for all analyzed surfaces (p < 0.05). On day 4, IL1β levels were significantly higher on bio-anodized compared to acid etched surfaces (p = 0.0097). TGFβ1 did not show differences among the surfaces at days 1 and 4. The responses of non-stimulated mononuclear cells to titanium surfaces suggest only modest effects of the surface treatment and roughness on pro-inflammatory cytokine (IL1β) release.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Titanio
/
Células Sanguíneas
/
Interleucina-1beta
/
Factor de Crecimiento Transformador beta1
Tipo de estudio:
Estudios de evaluación
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. oral res
Asunto de la revista:
Odontología
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Uberlândia/BR
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