Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
Biol. Res
;
44(3): 243-249, 2011. ilus
Artículo
en Inglés
| LILACS
| ID: lil-608620
ABSTRACT
Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine assay. In addition, the expression of Hsp-27 mRNA detected by RT-PCR and Hsp-27 protein detected by immnofluorescence was present in all conditions, except when using the highest concentration of Cimicifuga foetida extract (2,000 jig /L). We conclude that Hsp 27 expression at 2,000 jig /L Cimicifuga foetida extract is diminished. This is the first report showing the Hsp-27 expression after exposure to Cimicifuga foetida extract in MCF-7 cells.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Neoplasias de la Mama
/
Extractos Vegetales
/
Cimicifuga
/
Fitoterapia
/
Antineoplásicos Fitogénicos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Chile
/
Francia
/
Estados Unidos
Institución/País de afiliación:
Naturex SA/FR
/
Naturex, Inc/US
/
Universidad de Valparaíso/CL
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