A direct method for cDNA cloning employing translation released mRNA
Braz. j. med. biol. res
;
20(3/4): 313-20, 1987. ilus
Artículo
en Inglés
| LILACS
| ID: lil-61007
RESUMO
1. A new cloning procedure is described for cDNA synthesis from mRNA released by in vitro translation of polysomes in a cell-free amino acid incorporating system. The usefulness of the method lies in the feasibility of employing nanogram amounts of mRNA. 2. Complementary DNA is synthesized derectly in the translation mixture simply by adjusting the concentration of some components and removing ribosomes by boiling and centrifugation. 3. As an example, we report here the construction and characterization of cDNA clone corresponding to chick alfa (1) procollagen starting from a collagen-synthesizing polysome fraction obtained from chick embryos
Buscar en Google
Índice:
LILACS (Américas)
Asunto principal:
Polirribosomas
/
Técnicas In Vitro
/
ADN Recombinante
/
ARN Mensajero
/
Clonación Molecular
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1987
Tipo del documento:
Artículo
/
Documento de proyecto
Similares
MEDLINE
...
LILACS
LIS