Investigation into in vitro anti-leishmanial combinations of calcium channel blockers and current anti-leishmanial drugs
Mem. Inst. Oswaldo Cruz
;
106(8): 1032-1038, Dec. 2011. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-610982
ABSTRACT
The need for drug combinations to treat visceral leishmaniasis (VL) arose because of resistance to antimonials, the toxicity of current treatments and the length of the course of therapy. Calcium channel blockers (CCBs) have shown anti-leishmanial activity; therefore their use in combination with standard drugs could provide new alternatives for the treatment of VL. In this work, in vitro isobolograms of Leishmania (Leishmania) chagasi using promastigotes or intracellular amastigotes were utilised to identify the interactions between five CCBs and the standard drugs pentamidine, amphotericin B and glucantime. The drug interactions were assessed with a fixed ratio isobologram method and the fractional inhibitory concentrations (FICs), sum of FICs (ΣFICs) and the overall mean ΣFIC were calculated for each combination. Graphical isobologram analysis showed that the combination of nimodipine and glucantime was the most promising in amastigotes with an overall mean ΣFIC value of 0.79. Interactions between CCBs and the anti-leishmanial drugs were classified as indifferent according to the overall mean ΣFIC and the isobologram graphic analysis.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Pentamidina
/
Bloqueadores de los Canales de Calcio
/
Anfotericina B
/
Macrófagos Peritoneales
/
Leishmania
/
Leishmaniasis Visceral
/
Antiprotozoarios
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Adolfo Lutz/BR
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