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Angiotensin II type 1 receptor blockade partially attenuates hypoxia-induced pulmonary hypertension in newborn piglets: relationship with the nitrergic system
Camelo Júnior, J. S; Martins, A. R; Rosa, E; Ramos, S. G; Hehre, D; Bancalari, E; Suguihara, C.
Afiliación
  • Camelo Júnior, J. S; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Puericultura e Pediatria. Ribeirão Preto. BR
  • Martins, A. R; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto. BR
  • Rosa, E; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto. BR
  • Ramos, S. G; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Patologia. Ribeirão Preto. BR
  • Hehre, D; , University of Miami Miller School of Medicine. Neonatal Developmental Biology Laboratory. Division of Neonatology. Department of Pediatrics. Miami. US
  • Bancalari, E; , University of Miami Miller School of Medicine. Neonatal Developmental Biology Laboratory. Division of Neonatology. Department of Pediatrics. Miami. US
  • Suguihara, C; , University of Miami Miller School of Medicine. Neonatal Developmental Biology Laboratory. Division of Neonatology. Department of Pediatrics. Miami. US
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;45(2): 163-171, Feb. 2012. ilus, tab
Article en En | LILACS | ID: lil-614574
Biblioteca responsable: BR1.1
ABSTRACT
The objective of this study was to observe possible interactions between the renin-angiotensin and nitrergic systems in chronic hypoxia-induced pulmonary hypertension in newborn piglets. Thirteen chronically instrumented newborn piglets (6.3 ± 0.9 days; 2369 ± 491 g) were randomly assigned to receive saline (placebo, P) or the AT1 receptor (AT1-R) blocker L-158,809 (L) during 6 days of hypoxia (FiO2 = 0.12). During hypoxia, pulmonary arterial pressure (Ppa; P < 0.0001), pulmonary vascular resistance (PVR; P < 0.02) and the pulmonary to systemic vascular resistance ratio (PVR/SVR; P < 0.05) were significantly attenuated in the L (N = 7) group compared to the P group (N = 6). Western blot analysis of lung proteins showed a significant decrease of endothelial NOS (eNOS) in both P and L animals, and of AT1-R in P animals during hypoxia compared to normoxic animals (C group, N = 5; P < 0.01 for all groups). AT1-R tended to decrease in L animals. Inducible NOS (iNOS) did not differ among P, L, and C animals and iNOS immunohistochemical staining in macrophages was significantly more intense in L than in P animals (P < 0.01). The vascular endothelium showed moderate or strong eNOS and AT1-R staining. Macrophages and pneumocytes showed moderate or strong iNOS and AT1-R staining, but C animals showed weak iNOS and AT1-R staining. Macrophages of L and P animals showed moderate and weak AT2-R staining, respectively, but the endothelium of all groups only showed weak staining. In conclusion, pulmonary hypertension induced by chronic hypoxia in newborn piglets is partially attenuated by AT1-R blockade. We suggest that AT1-R blockade might act through AT2-R and/or Mas receptors and the nitrergic system in the lungs of hypoxemic newborn piglets.
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Texto completo: 1 Índice: LILACS Asunto principal: Tetrazoles / Óxido Nítrico Sintasa / Bloqueadores del Receptor Tipo 1 de Angiotensina II / Hipertensión Pulmonar / Imidazoles / Hipoxia / Antihipertensivos Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Asunto de la revista: BIOLOGIA / MEDICINA Año: 2012 Tipo del documento: Article

Texto completo: 1 Índice: LILACS Asunto principal: Tetrazoles / Óxido Nítrico Sintasa / Bloqueadores del Receptor Tipo 1 de Angiotensina II / Hipertensión Pulmonar / Imidazoles / Hipoxia / Antihipertensivos Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Asunto de la revista: BIOLOGIA / MEDICINA Año: 2012 Tipo del documento: Article