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Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
Naskar, Soumen; Deb, Sitangsu M; Niranjan, Saket K; Kumar, Subodh; Sharma, Deepak; Sakaram, Durgam; Sharma, Arjava.
  • Naskar, Soumen; National Research Centre on Pig. Guwahati. IN
  • Deb, Sitangsu M; Institute Regional Research Station. Central Arid Zone Research. Pali-Marwar. IN
  • Niranjan, Saket K; National Bureau of Animal Genetic Resources. IN
  • Kumar, Subodh; s.af
  • Sharma, Deepak; Pt. Deen Dayal Upadhayaya Veterinary and Animal Sciences University. Mathura. IN
  • Sakaram, Durgam; Sri Venkateswara Veterinary University. Hyderabad. IN
  • Sharma, Arjava; Project Directorate on Cattle. Meerut. IN
Genet. mol. biol ; 35(1): 95-98, 2012. ilus, tab
Artículo en Inglés | LILACS | ID: lil-616999
ABSTRACT
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Búfalos / ADN Complementario / Diclororribofuranosil Benzoimidazol Límite: Animales Idioma: Inglés Revista: Genet. mol. biol Asunto de la revista: Genética Año: 2012 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Institute Regional Research Station/IN / National Bureau of Animal Genetic Resources/IN / National Research Centre on Pig/IN / Project Directorate on Cattle/IN / Pt. Deen Dayal Upadhayaya Veterinary and Animal Sciences University/IN / Sri Venkateswara Veterinary University/IN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Búfalos / ADN Complementario / Diclororribofuranosil Benzoimidazol Límite: Animales Idioma: Inglés Revista: Genet. mol. biol Asunto de la revista: Genética Año: 2012 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Institute Regional Research Station/IN / National Bureau of Animal Genetic Resources/IN / National Research Centre on Pig/IN / Project Directorate on Cattle/IN / Pt. Deen Dayal Upadhayaya Veterinary and Animal Sciences University/IN / Sri Venkateswara Veterinary University/IN