Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
Mem. Inst. Oswaldo Cruz
;
107(4): 510-512, June 2012. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-626445
ABSTRACT
Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Paracoccidioides
/
Paracoccidioidomicosis
/
Manejo de Especímenes
/
Pruebas de Fijación de Látex
/
Anticuerpos Antifúngicos
Tipo de estudio:
Estudio diagnóstico
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2012
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal do Pará/BR
Similares
MEDLINE
...
LILACS
LIS