Development of a sensitive enzyme immunoassay (ELISA) for specific identification of Lachesis acrochorda venom
J. venom. anim. toxins incl. trop. dis
;
18(2): 173-179, 2012. ilus, graf
Artículo
en Inglés
| LILACS, VETINDEX
| ID: lil-639476
ABSTRACT
The snake genus Lachesis provokes 2 to 3% of snakebites in Colombia every year. Two Lachesis species, L. acrochorda and L. muta, share habitats with snakes from another genus, namely Bothrops asper and B. atrox. Lachesis venom causes systemic and local effects such as swelling, hemorrhaging, myonecrosis, hemostatic disorders and nephrotoxic symptoms similar to those induced by Bothrops, Portidium and Bothriechis bites. Bothrops antivenoms neutralize a variety of Lachesis venom toxins. However, these products are unable to avoid coagulation problems provoked by Lachesis snakebites. Thus, it is important to ascertain whether the envenomation was caused by a Bothrops or Lachesis snake. The present study found enzyme linked immunosorbent assay (ELISA) efficient for detecting Lachesis acrochorda venom in a concentration range of 3.9 to 1000 ng/mL, which did not show a cross-reaction with Bothrops, Portidium, Botriechis and Crotalus venoms. Furthermore, one fraction of L. acrochorda venom that did not show crossreactivity with B. asper venom was isolated using the same ELISA antibodies; some of its proteins were identified including one Gal-specific lectin and one metalloproteinase. This test may be useful to physicians, since it could be applicable for tracking the kinetic distribution of antigens in patients or experimentally envenomed animals.(AU)
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Venenos de Serpiente
/
Ensayo de Inmunoadsorción Enzimática
/
Técnicas para Inmunoenzimas
/
Viperidae
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Año:
2012
Tipo del documento:
Artículo
Institución/País de afiliación:
University of Antioquia/CO
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